MicroRNAs in Diffuse Large B-Cell Lymphoma (DLBCL): Biomarkers with Prognostic Potential

MicroRNAs in Diffuse Large B-Cell Lymphoma (DLBCL): Biomarkers with Prognostic Potential Full article

Journal

Cancers
ISSN: 2072-6694

Output data

Year: 2025, Volume: 17, Number: 8, Article number : 1300, Pages count : 18 DOI: 10.3390/cancers17081300

Tags

microRNA; diffuse large B-cell lymphoma; biomarkers

Authors

Veryaskina Yuliya A. ^1,2 , Titov Sergei E. ^3,2 , Kovynev Igor B. ⁴ , Fyodorova Sofya S. ⁴ , Berezina Olga V. ⁴ , Zhurakovskij Igor P. ⁴ , Antonenko Oksana V. ² , Demakov Sergei A. ² , Demenkov Pavel S. ⁵ , Ruzankin Pavel S. ^6,7 , Tarasenko Anton S. ^6,7 , Pospelova Tatiana I. ⁴ , Zhimulev Igor F. ²

Affiliations

1	Laboratory of Gene Engineering, Institute of Cytology and Genetics, SB RAS, Novosibirsk 630090, Russia
2	Laboratory of Molecular Genetics, Department of the Structure and Function of Chromosomes, Institute of Molecular and Cellular Biology, SB RAS, Novosibirsk 630090, Russia
3	AO Vector-Best, Novosibirsk 630117, Russia
4	Department of Therapy, Hematology and Transfusiology, Novosibirsk State Medical University, Novosibirsk 630091, Russia
5	Laboratory of Computer Proteomics, Institute of Cytology and Genetics, SB RAS, Novosibirsk 630090, Russia
6	Department of Probability Theory and Mathematical Statistics, Novosibirsk State University, Novosibirsk 630090, Russia
7	Sobolev Institute of Mathematics, Novosibirsk 630090, Russia

Funding (5)

1	Russian Science Foundation	20-14-00074-П
2	Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences	FWNR-2022-0020
3	Department of the Structure and Function of Chromosomes, Laboratory of Molecular Genetics Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia	FWGZ-2021-0014
4	Sobolev Institute of Mathematics	FWNF-2022-0010
5	Sobolev Institute of Mathematics	FWNF-2024-0001

Background/Objectives: The heterogeneity of diffuse large B-cell lymphoma (DLBCL) based on differences in both genetic and epigenetic factors contributes to the dynamics of tumor growth and efficacy of cytoreductive therapy, as well as considerably affecting disease prognosis. This study aimed to detect microRNAs (miRNAs) capable of improving prognostic accuracy in DLBCL patients. Methods: We performed miRNA sequencing in bone marrow (BM) samples collected from DLBCL patients. Next, the expression levels of miRNAs in lymph node (LN) samples (n = 43) and BM samples (n = 70) were analyzed by real-time RT-PCR in the group of DLBCL patients. Results: It was found that the expression levels of miRNA-10b, -100, -125a, -125b, -126, -143, -23a and let-7a were statistically significantly reduced in the group of DLBCL patients who had a poor prognosis compared to DLBCL patients with a favorable prognosis (p < 0.05). Kaplan–Meier survival analysis demonstrated that the upregulated expression of miRNA-23a, miRNA-125a, and miRNA-100 was associated with better overall survival in DLBCL patients. A statistically significant elevation in the expression levels of miRNA-151a, miRNA-148b and miRNA-192 in the BM samples was observed for DLBCL patients both with and without BM involvement compared to BM samples from non-cancerous blood disease (NCBD) patients (p < 0.05). Statistically significant upregulation of PD-L1, TIMP1, TOP2A, and TP53 was observed in BM samples from DLBCL patients with and without BM involvement in comparison with BM samples from NCBD patients (p < 0.05). Conclusions: miRNA-23a, miRNA-125a, and miRNA-100 were shown to be potential prognostically significant biomarkers in DLBCL patients. Changes in expression levels of miRNA-151a, miRNA-148b, miRNA-192, PD-L1, TIMP1, TOP2A, and TP53 reflect alterations in the BM without morphological or immunophenotypic signs of a DLBCL-related BM patholog

Veryaskina Y.A. , Titov S.E. , Kovynev I.B. , Fyodorova S.S. , Berezina O.V. , Zhurakovskij I.P. , Antonenko O.V. , Demakov S.A. , Demenkov P.S. , Ruzankin P.S. , Tarasenko A.S. , Pospelova T.I. , Zhimulev I.F.
MicroRNAs in Diffuse Large B-Cell Lymphoma (DLBCL): Biomarkers with Prognostic Potential
Cancers. 2025. V.17. N8. 1300 :1-18. DOI: 10.3390/cancers17081300 WOS Scopus OpenAlex

Submitted:	Mar 4, 2025
Accepted:	Apr 9, 2025
Published print:	Apr 12, 2025
Published online:	Apr 12, 2025

Web of science:	WOS:001475898800001
Scopus:	2-s2.0-105003407610
OpenAlex:	W4409439856

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