Locus-Specific Bisulfate NGS Sequencing of GSTP1, RNF219, and KIAA1539 Genes in the Total Pool of Cell-Free and Cell-Surface-Bound DNA in Prostate Cancer: A Novel Approach for Prostate Cancer Diagnostics | Sciact - Система учета научной деятельности ИМ СО РАН

Locus-Specific Bisulfate NGS Sequencing of GSTP1, RNF219, and KIAA1539 Genes in the Total Pool of Cell-Free and Cell-Surface-Bound DNA in Prostate Cancer: A Novel Approach for Prostate Cancer Diagnostics Научная публикация

Журнал

Cancers
ISSN: 2072-6694

Вых. Данные

Год: 2023, Том: 15, Номер: 2, Номер статьи : 431, Страниц : 18 DOI: 10.3390/cancers15020431

Ключевые слова

cell-free DNA; cell-surface-bound DNA; methylation; locus-specific NGS sequencing; prostate cancer

Авторы

Bryzgunova Olga ¹ , Bondar Anna ¹ , Ruzankin Pavel ^2,3 , Tarasenko Anton ^2,3 , Zaripov Marat ¹ , Kabilov Marsel ¹ , Laktionov Pavel ¹

Организации

1	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch Russian Academy of Sciences
2	Sobolev Institute of Mathematics, Siberian Branch Russian Academy of Sciences
3	Department of Mathematics and Mechanics, Novosibirsk State University

Информация о финансировании (2)

1	Институт математики им. С.Л. Соболева СО РАН	FWNF-2022-0010
2	Институт Химической Биологии И Фундаментальной Медицины СО РАН	121030200173-6

The locus-specific methylation of three genes (GSTP1, RNF219, and KIAA1539, also known as FAM214B) in the total pool of blood cell-free DNA, including cell-free DNA from plasma and cell-surface-bound DNA, of patients with prostate cancer and healthy donors was studied on the MiSeq platform. Our study found a higher methylation index of loci for total cell-free DNA compared with cell-free DNA. For total cell-free DNA, the methylation of GSTP1 in each of the 11 positions provided a complete separation of cancer patients from healthy donors, whereas for cell-free DNA, there were no positions in the three genes allowing for such separation. Among the prostate cancer patients, the minimum proportion of GSTP1 genes methylated in any of the 17 positions was 12.1% of the total circulated DNA fragments, and the minimum proportion of GSTP1 genes methylated in any of the 11 diagnostically specific positions was 8.4%. Total cell-free DNA was shown to be more convenient and informative as a source of methylated DNA molecules circulating in the blood than cell-free DNA.

Bryzgunova O. , Bondar A. , Ruzankin P. , Tarasenko A. , Zaripov M. , Kabilov M. , Laktionov P.
Locus-Specific Bisulfate NGS Sequencing of GSTP1, RNF219, and KIAA1539 Genes in the Total Pool of Cell-Free and Cell-Surface-Bound DNA in Prostate Cancer: A Novel Approach for Prostate Cancer Diagnostics
Cancers. 2023. V.15. N2. 431 :1-18. DOI: 10.3390/cancers15020431 WOS Scopus РИНЦ OpenAlex

Поступила в редакцию:	18 нояб. 2022 г.
Опубликована в печати:	9 янв. 2023 г.
Опубликована online:	9 янв. 2023 г.

Web of science:	WOS:000914549700001
Scopus:	2-s2.0-85146742642
РИНЦ:	60567234
OpenAlex:	W4313897989

БД	Цитирований
Scopus	1
OpenAlex	1