Effect of Preservation on Changes in the Volume of Corneal Endothelial Cells in an Environment with a High Concentration of Potassium | Sciact - Система учета научной деятельности ИМ СО РАН

Effect of Preservation on Changes in the Volume of Corneal Endothelial Cells in an Environment with a High Concentration of Potassium Научная публикация

Журнал

Journal of evolutionary biochemistry and physiology
ISSN: 0022-0930 , E-ISSN: 1608-3202

Вых. Данные

Год: 2024, Том: 60, Номер: 4, Страницы: 1669-1675 Страниц : 7 DOI: 10.1134/s0022093024040331

Ключевые слова

corneal endothelium, corneal transplantation, hypothermic preservation

Авторы

Katkova L.E. ¹ , Baturina G.S. ^1,2 , Teterin M.M. ³ , Sakhanenko A.I. ⁴ , Palchikova I.G. ^2,5 , Iskakov I.A. ⁶ , Solenov E.I. ^1,2,3

Организации

1	Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences
2	Novosibirsk State University
3	Novosibirsk State Technical University
4	Sobolev Institute of Mathematics of the Siberian Branch of the Russian Academy of Sciences
5	Technological Design Institute of Scientific Instrument Engineering of the Siberian Branch of the Russian Academy of Sciences
6	The S.Fyodorov Eye Microsurgery Federal State Institution

Информация о финансировании (2)

1	Институт математики им. С.Л. Соболева СО РАН	FWNF-2022-0010
2	Министерство науки и высшего образования РФ	FWNR-2022-0019

The experimental study of preservation length on the effect of high potassium concentration in the medium on the volume of human corneal endothelial cells was done. The results of the study of individual samples of fragments of donor material and the values calculated using joined data after hypothermic preservation for 4 and 10 days are presented. The increase of the time when cornea samples are kept in preservation medium (Eusol-C) at 4°C led to a decrease in the average value (M ± SEM) of cell swelling indicator (Q) in a potassium medium from 1.055 ± 0.001; n = 982 to 1.014 ± 0.001; n = 338; after 4 and 10 days, respectively. Student’s t-test for independent samples showed a high degree of significance for the difference between these values (p = 2E-76). Identification of the proportion of cells capable to swell in a medium with a high content of potassium ions (Q ≥ 1), reflecting the electrogenic activity in these cells, showed a decrease of this indicator in the studied groups with increasing duration of preservation (94.3% and 56.8% after 4 and 10 days, respectively). Based on the results of the study, it is suggested that the values of endothelial cells swelling in a potassium environment can serve as indicators of the cells’ ability to restore electrogenic transport. It is concluded that the study of cell swelling in a medium with a high content of potassium ions can provide information for predicting the functionality of the graft.

Katkova L.E. , Baturina G.S. , Teterin M.M. , Sakhanenko A.I. , Palchikova I.G. , Iskakov I.A. , Solenov E.I.
Effect of Preservation on Changes in the Volume of Corneal Endothelial Cells in an Environment with a High Concentration of Potassium
Journal of evolutionary biochemistry and physiology. 2024. V.60. N4. P.1669-1675. DOI: 10.1134/s0022093024040331 WOS РИНЦ OpenAlex

Katkova L.E. , Baturina G.S. , Teterin M.M. , Sakhanenko A.I. , Palchikova I.G. , Iskakov I.A. , Solenov E.I.
Влияние консервации на изменение объема клеток эндотелия роговицы в среде с высокой концентрацией калия
Российский физиологический журнал им. И.М. Сеченова. 2024. V.110. N8. P.1264-1272. DOI: 10.31857/s0869813924080041 РИНЦ OpenAlex

Поступила в редакцию:	27 мая 2023 г.
Опубликована в печати:	18 июл. 2024 г.
Принята к публикации:	26 июл. 2024 г.
Опубликована online:	29 авг. 2024 г.

Web of science:	WOS:001303635800013
РИНЦ:	69095739
OpenAlex:	W4402001310

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